Vincent L. Chiang

Distinguished Professor for Natural Resources Innovation

College of Natural Resources

Department of Forestry and Environmental Resources

Forest Biotechnology Group

North Carolina State University

Campus Box 7247

Raleigh, NC 27695-7247, USA

Telephone: 919/513-0098

E-Mail: vchiang@ncsu.edu

Education

B.S., 1976, College of Chinese Culture, Taiwan (Chem. Engr. Pulp and Paper)

M.S., 1980, University of Washington (Chemistry)

Ph.D., 1983, University of Washington (Chemistry and Biochemistry)

Experience

2008-Present North Carolina State University, Jordan Family Distinguished Professor for Natural Resources Innovation

2018-Present Director

State Key Laboratory of Tree Genetics and Breeding,

Northeast Forestry University, Harbin, China

2014-2017  Distinguished Visiting Professor

State Key Laboratory of Tree Genetics and Breeding,

Northeast Forestry University, Harbin, China

2002-2017 Professor and Co-Director,

Forest Biotechnology Group, North Carolina State University

2015-Present  Dingying Chair Professor

South China Agricultural University

2004-2014  Director,

Forest Biotechnology Industrial Research Consortium

North Carolina State University

2007-2014  Scientific Advisory Board Member,

Agriculture Biotechnology Research Center

Academia Sinica, Taipei, Taiwan

2011 & 2012  Visiting Professor,

National Taiwan University, Taipei, Taiwan

2006-2009:  Board of Directors,

Institute of Forest Biotechnology, Raleigh, North Carolina

2001 Spring  Distinguished Visiting Professor,

Japanese Society for the Promotion of Science

Kyoto University, Kyoto, Japan

1995-2002:  Director,

Plant Biotechnology Research Center

Michigan Technological University

1992-2002:  Professor,

School of Forestry, Michigan Technological University

1988-1992:  Associate Professor, School of Forestry, Michigan Technological University

1987-2002:  Adjunct Professor, Department of Biological Sciences

Michigan Technological University

1985-1988:  Assistant Professor, School of Forestry, Michigan Technological University

1983-1985: Postdoc Research Associate, University of Washington, Seattle, Washington

Honors and Awards

— Fellow, American Association for the Advancement of Science

— Fellow, International Academy of Wood Science

— North Carolina State University Outstanding Research Award

— Michigan Technological University Distinguished Faculty Research Award

— Distinguished Faculty Member Award

  of the Michigan Association of Governing Boards of State Universities

— NSF Biotechnology Program Biofuels panelist

— DOE BioEnergy Science Program panelist

— USDA National Research Initiative Competitive Grants panelist

— Advisory Board of the Journal of Wood Chemistry and Technology

— Executive Editor of the Taiwan Journal of Forest Science

— International Advisory Board of the Journal of Wood Science

DEMONSTRATED LEADERSHIP

Overall

— Over 15 years’ experience in managing research centers and providing intellectual leadership to center members that consist of faculty members, post-docs, and graduate students, for multi- and interdisciplinary research in biochemistry, molecular biology, and biotechnology;

— Established the first biotech research center, the Plant Biotech Research Center (PBRC), at Michigan Tech. in 1995;

— Managed up to 20 research personnel, including up to 3 tenure-tracked faculty members, as the Director of PBRC at MTU from 1995 to 2002;

— Secured and managed research funding for the Center with an annual research expenditure of approximately USD $1 million;

— Managing up to 20 research and administrative personnel, as a Co-Director of Forest Biotech Group at NCSU since 2002;

— Established the NCSU Forest Biotechnology Industrial Research Consortium (FORBIRC) and recruited up to 13 biotech companies worldwide and 15 faculty members since 2004;

— Managing all FORBIRC research activities and established a competitive grant source from the Consortium membership dues to support 4-5 research projects/yr for the Consortium faculty;

— Securing and managing research funding for Forest Biotech Group with an annual research expenditure of approximately USD $2 million since 2002;

— Organized and managing multi-million dollar plant genome research programs funded by the US National Science Foundation and US Department of Energy with a total of 37 research personnel.

MAJOR RESEARCH ACCOMPLISHMENTS

Overall

— Total research grants received: USD $30,190,168 ($20,108,503 since 2002 at NCSU)

— Total number of Post-Doctoral Associates directly supervised: 20 (since 2002 at NCSU)

— Total number of Senior Research Associates directly supervised: 5 (since 2002 at NCSU)

— Total number of Graduate Students directly supervised: 12 Ph.D. and 10 MS (since 2002 at NCSU)

— Total number of Visiting Professor/Scientist directly supervised: 12 (since 2002 at NCSU)

— Total number of Lab Managers and Technicians directly supervised: 6 (since 2002 at NCSU)

— Total number of refereed publications: 119 (72 since 2002 at NCSU)

— The first to demonstrate genetic downregulation of lignin biosynthesis in tree species—

   a Nature Biotech cover story

— Total number of Plenary and Keynote Lectures: 32 (27 since 2002 at NCSU)

— Total number of US and Europe patents allowed: 21

Publications

1. Hao Chen, Jack P. Wang, Huizi Liu, Huiyu Li, Ying-Chung Jimmy Lin, Rui SHI, Chenmin Yang, Jinghui Gao, Chenguang Zhou, Quanzi Li, Ronald R. Sederoff, Wei Li, and Vincent L. Chiang* (2019). Hierarchical Transcription-Factor and Chromatin Binding Network for Wood Formation in Populus trichocarpa. Plant Cell. 31: 602–626. (Cover story)

2. Xiaojing Yan, Jie Liu, Hoon Kim, Baoguang Liu, Xiong Huang, Zhichang Yang, Ying‐Chung Jimmy Lin, Hao Chen, Chenmin Yang, Jack P. Wang, David C. Muddiman, John Ralph, Ronald R. Sederoff, Quanzi Li*, and Vincent L. Chiang* (2019). CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa. New Phytologist. 222(1): 244-260.

3. Wang JP, Liu B, Sun Y, Chiang VL, Sederoff RR (2019). Enzyme-Enzyme Interactions in Monolignol Biosynthesis. Frontiers in Plant Science. 11; 9:1942.

4. Naik P, Wang JP, Sederoff R, Chiang VL, Williams C, Ducoste JJ (2018). Assessing the impact of the 4CL enzyme complex on the robustness of monolignol biosynthesis using metabolic pathway analysis. PLoS One. 6; 13(3):e0193896.

5. Jack P. Wang, Megan L. Matthews, Punith P. Naik, Cranos M. Williams, Joel J. Ducoste, Ronald R. Sederoff and Vincent L. Chiang* (2018). Flux modeling for monolignol biosynthesis. Current Opinion in Biotechnology. 56:187-192.

6. Shuang Li, Ying-Chung Jimmy Lin, Pengyu Wang, Baofeng Zhang, Meng Li, Su Chen, Rui Shi, Sermsawat Tunlaya-Anukit, Xinying Liu, Zhifeng Wang, Xiufang Dai, Jing Yu, Chenguang Zhou, Baoguang Liu, Jack P. Wang, Vincent L. Chiang* and Wei Li* (2018). Histone Acetylation Cooperating with AREB1 Transcription Factor Regulates Drought Response and Tolerance in Populus trichocarpa. Plant Cell. 31: 663–686.

7. Wang JP, Matthews ML, Williams CM, Shi R, Yang C, Tunlaya-Anukit S, Chen HC, Li Q, Liu J, Lin CY, Naik P, Sun YH, Loziuk PL, Yeh TF, Kim H, Gjersing E, Shollenberger T, Shuford CM, Song J, Miller Z, Huang YY, Edmunds CW, Liu B, Sun Y, Lin YJ, Li W, Chen H, Peszlen I, Ducoste JJ, Ralph J, Chang HM, Muddiman DC, Davis MF, Smith C, Isik F, Sederoff R, Chiang VL* (2018). Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis. Nature Communications. 9:1579.

8. Lin YJ, Chen H, Li Q, Li W, Wang JP, Shi R, Tunlaya-Anukit S, Shuai P, Wang Z, Ma H, Li H, Sun YH, Sederoff RR, Chiang VL (2017). Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa. Proceedings of the National Academy of Sciences of the United States of America. 114: E9722-E9729.

9. Jack P. Wang, Sermsawat Tunlaya-Anukit, Rui Shi, Ting-Feng Yeh, Chuang, Ling, Fikret Isik, Chenmin Yang, Jie Liu, Quanzi Li, Philip Loziuk, Punith P. Naik, David C. Muddiman, Joel J. Ducoste, Cranos M. Williams, Ronald R. Sederoff, and Vincent L. Chiang (2017). A Proteomic-Based Quantitative Analysis of the Relationship Between Monolignol Biosynthetic Protein Abundance and Lignin Content Using Transgenic Populus trichocarpa. In Recent Advances in Polyphenol Research, John Wiley & Sons, pp. 89-107.

10. Blumer-Schuette SE, Zurawski JV, Conway JM, Khatibi P, Lewis DL, Li Q, Chiang VL, Kelly RM (2017). Caldicellulosiruptor saccharolyticus transcriptomes reveal consequences of chemical pretreatment and genetic modification of lignocellulose. Microbial Biotechnology. 10:1546-1557.

11. Rui Shi, Jack P. Wang, Ying-Chung Lin, Quanzi Li, Ying-Hsuan Sun, Hao Chen, Ronald R. Sederoff, and Vincent L. Chiang (2017). Tissue and cell-type co-expression networks of transcription factors and wood component genes in Populus trichocarpa. Planta 245:927-938.

12. Chien-Yuan Lin, Quanzi Li, Sermsawat Tunlaya-Anukit, Rui Shi, Ying-Hsuan Sun,  Jack P. Wang, Jie Liu, Philip Loziuk, Warren C. Edmunds, Zachary D. Miller, Illona Peszlen, David C. Muddiman, D. C., Ronald R. Sederoff, and Vincent L. Chiang (2016). A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa. Tree Genetics & Genomes, 12, 1-18.

13. Sapna Kumari, Wenping Deng, Chathura Gunasekara, Vincent L. Chiang, Huann-Sheng Chen, Hao Ma, Xin Davis, Hairong Wei (2016). Bottom-up GGM algorithm for constructing multilayered hierarchical gene regulatory networks that govern biological pathways or processes. BMC Bioinformatics 17:132.

14. Quanzi Li, Ting-Feng Yeh, Chenmin Yang, Jingyuan Song, Zenn-Zong Chen, Ronald R. Sederoff, and Vincent L. Chiang (2015) Populus trichocarpa, in "Agrobacterium Protocols" Methods in Mol. Biol. 1224: 357-363.

15. Philip L. Loziuk, Jennifer Parker, Wei Li, Chien-Yuan Lin, Jack P. Wang, Quanzi Li, Ronald R. Sederoff, Vincent L. Chiang, and David C. Muddiman (2015). Elucidation of Xylem-Specific Transcription Factors and Absolute Quantification of Enzymes Regulating Cellulose Biosynthesis in Populus trichocarpa. Journal of Proteome Research. 144158-4168.

16. Chien-Yuan Lin, Jack P. Wang, Quanzi Li, Hsi-Chuan Chen, Jie Liu, Philip Loziuk, Jina Song, Cranos Williams, David C. Muddiman, Ronald R. Sederoff, and Vincent L. Chiang. (2015). 4-Coumaroyl and Caffeoyl Shikimic Acids Inhibit 4-Coumaric Acid: Coenzyme A Ligases and Modulate Metabolic Flux for 3-Hydroxylation in Monolignol Biosynthesis of Populus trichocarpa. Molecular Plant. 8:176-187.

17. Jack P. Wang, Ling Chuang, Philip L. Loziuk, Hao Chen, Ying-Chung Lin, Rui Shi, Guan-Zheng Qu, David C. Muddiman, Ronald R. Sederoff, and Vincent L. Chiang (2015). Phosphorylation is an on/off switch for 5-hydroxyconiferaldehyde O-methyltransferase activity in poplar monolignol biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 112:8481-8486.

18. Philip L. Loziuk, Ronald R. Sederoff, Vincent L. Chiang and David C. Muddiman (2014) Establishing ion ration thresholds based on absolute peak area for absolute protein quantification using protein cleavage isotope dilution mass spectrometry. Analyst 139: 5450.

19. Suvi Sutela, Terhi Hahl, Heidi Tiimonen, Tuija Aronen, Tiina Ylioja, Tapio Laakso, Pekka Saranpää, Vincent L. Chiang, Riitta Julkunen-Tiitto, Hely Häggman (2014). Phenolic Compounds and Expression of 4CL Genes in Silver Birch Clones and Pt4CL1a Lines. PLOS ONE 9e114434.

20. Kabindra Kafle, Rui Shi, Christopher M. Lee, Ashutosh Mittal, Yong Bum Park, Ying-Hsuan Sun, Sunkyu Park, Vincent L. Chiang, and Seong H. Kim (2014). Vibrational sum-frequency-generation (SFG) spectroscopy study of the structural assembly of cellulose microfibrils in reaction wood. Cellulose, 21:2219-2231.

21. Quanzi Li, Jian Song, Shaobing Peng, Jack P. Wang, Guan-Zheng Qu, Ronald R. Sederoff, and Vincent L. Chiang. (2014). Plant biotechnology for lignocellulosic biofuel production. Plant Biotechnology Journal, 12:1174-1192 (the Cover Story).

22. Tomoyuki Nakatsubo, Safendrri Komara Ragamustari, Takefumi Hattori, Eiichiro Ono, Masaomi Yamamura, Laigeng Li, Vincent L. Chiang, Toshiaki Umezawa (2014). A new O-methyltransferase for monolignol synthesis in Carthamus tinctorius. Plant Biotechnology Journal. 31:545-553.

23. Wei Li, Ying-Chung Lin, Quanzi Li, Rui Shi, Chien-Yuan Lin, Hao Chen, Ling Chuang, Guan-Zheng Qu, Ronald Sederoff, and Vincent L. Chiang. (2014). A robust chromatin immunoprecipitation protocol for studying transcription factor-DNA interactions and histone modifications in wood-forming tissue. Nature Protocols. 9:2180-2193.

24. Ying-Chung Lin, Wei Li, Hao Chen, Quanzi Li, Ying-Hsuan Sun, Rui Shi, Chien-Yuan Lin, Jack Wang, Hsi-Chuan Chen, Ling Chuang, Guan-Zheng Qu, Ronald Sederoff, and Vincent L. Chiang. (2014). A simple improved-throughput xylem protoplast system for studying wood formation. Nature Protocols. 9:2194-2205.

25. Hsi-Chuan Chen, Jina Song, Jack P. Wang, Ying-Chung Lin, Joel Ducoste, Christopher M. Shuford, Jie Liu, Quanzi Li, Rui Shi, Angelito Nepomuceno, Fikret Isik, David C. Muddiman, Cranos Williams, Ronald R. Sederoff, and Vincent L. Chiang. (2014). Systems biology of lignin biosynthesis in Populus trichocarpa: Heteromeric 4-coumaric acid: CoA ligase (4CL) protein complex formation, regulation, and numerical modeling. Plant Cell. 26: 876-893.

26. Jack P. Wang, Punith P. Naik, Hsi-Chuan Chen, Rui Shi, Chien-Yuan Lin, Jie Liu, Christopher M. Shuford, Quanzi Li, Cranos M. Williams, David C. Muddiman, Joel J. Ducoste, Ronald R. Sederoff, and Vincent L. Chiang. (2014). Complete proteomic-based enzyme reaction and inhibition kinetics reveal how monolignol biosynthetic enzyme families affect metabolic-flux and lignin. Plant Cell. 26: 894-914.

27. Xiang Li, Chathura Gunasekara, Yufeng Guo, Hang Zhang, Liang Lei, Sermsawat Tunlaya-Anukit, Victor Busov, Vincent L. Chiang, and Wei, Hairong. (2014). Pop’s Pipes: poplar gene expression data analysis pipelines. Tree Genetics & Genomes, 10: 1093-1101.

28. Amborella Genome Project. (2013). The Amborella Genome and the Evolution of Flowering Plants. Science 342: 1241089-10.

29. Ying-Chung Lin, Wei Li, Ying-Hsuan Sun, S. Kumari, Hairong Wei, Quanzi Li, Sermsawat Tunlaya-Anukit, Ronald R. Sederoff, and Vincent L. Chiang. (2013). SND1 transcription factor-directed quantitative functional hierarchical genetic regulatory network in wood formation in Populus trichocarpa. Plant Cell. 25: 4321-4341.

30. Philip Loziuk, Jack Wang, Quanzi Li, Ronnald Sederoff, Vincent Chiang, and David Muddiman. (2013). Understanding the role of proteolytic digestion on the discovery and targeted proteomic measurements using liquid chromatography-tandem mass spectrometry and design of experiments. Journal of Proteome Research. 12: 5820-5829.

31. Shanfa Lu, Quanzi Li, Hairong Wei, Mao-Ju Chang, Sermsawat Tunlaya-Anukit, Hoon Kim, Jie Liu, Jingyuan Song, Ying-Hsuan Sun, Lichai Yuan, Ting-Feng Yeh, John Ralph, Ronald R. Sederoff, and Vincent L. Chiang. (2013). Ptr-miR397a is a master negative regulator of laccase genes affecting lignin polymerization in Populus trichocarpa. Proceedings of the National Academy of Sciences of the United States of America. 110: 10848-10853.

32. Hsi-Chuan Chen, Jina Song, Cranos M. Williams, Christopher M. Shuford, Jie Liu, Jack P. Wang, Quanzi Li, Rui Shi, Emine Gokce, Joel Ducoste, David C. Muddiman, Ronald R. Sederoff, and Vincent L. Chiang. (2013). Monolignol Pathway 4-Coumaric Acid:Coenzyme A Ligases in Populus. trichocarpa: Novel Specificity, Metabolic Regulation, and Simulation of Coenzyme A Ligation Fluxes. Plant Physiology 161:1501-1516.

33. Rui Shi, Christopher M. Shuford, Jack P. Wang, Ying-Hsuan Sun, Zhichang Yang, Hsi-Chuan Chen, Sermsawat Tunlaya-Anukit, Quanzi Li, Jie Liu, David C. Muddiman, Ronald R. Sederoff, and Vincent L. Chiang (2013). Regulation of phenylalanine ammonia-lyase (PAL) gene family in wood-forming tissue of Populus trichocarpa. Planta 238:487-97.

34. Quanzi Li, Ying-Chung Lin, Ying-Hsuan Sun, Jian Song, Hao Chen, Xing-Hai Zhang, Ronald R. Sederoff, and Vincent L. Chiang. (2012). Splice variant of the SND1 transcription factor is a dominant negative of SND1 members and their regulation in Populus trichocarpa. Proceedings of the National Academy of Sciences of the United States of America. 109: 14699-14704.

35. Pradeep N. Perera, Martin Schmidt, Vincent L. Chiang, P. James Schuck,  and Paul D. Adams. (2012) Raman-spectroscopy-based noninvasive microanalysis of native lignin structure. Anal. Bioanal. Chem. 402:983-987.

36. Jie Liu, Rui Shi, Quanzi Li, Ronald R. Sederoff, Vincent L. Chiang. (2012). A standard reaction condition and a single HPLC separation system are sufficient for estimation of monolignol biosynthetic pathway enzyme activities. Planta 236:879-885.

37. Christopher M. Shuford, Ronald R. Sederoff, and Vincent L. Chiang, and David C. Muddiman. (2012). Peptide production and decay rates affect the quantitative accuracy of protein cleavage isotope dilution mass spectrometry (PC-IDMS). Mol Cell Proteomics 11:814-823.

38. Christopher Shuford, Quanzi Li, Ying-Hsuan Sun, Hsi-Chuan Chen, Jack P. Wang, Rui Shi, Ronald R. Sederoff, and Vincent L. Chiang, and David C. Muddiman. (2012). Comprehensive Quantification of Monolignol-pathway Enzymes in Populus trichocarpa by Protein Cleavage Isotope Dilution Mass Spectrometry. Journal of Proteome Research 11:3390-3404.

39. Jack P. Wang, Christopher M. Shuford,  Quanzi Li, Jina Song, Ying-Chung Lin, Ying-Hsuan Sun, Hsi-Chuan Chen, Cranos M. Williams, David C. Muddiman, Ronald R. Sederoff, Vincent L. Chiang. (2012). Functional Redundancy of the Two 5-Hydroxylases in Monolignol Biosynthesis of Populus trichocarpa: LC-MS/MS-Based Protein Quantification and Metabolic Flux Analysis. Planta 236:795-808.

40. Hsi-Chuan Chen, Quanzi Li, Christopher M. Shuford, Jie Liu, David C. Muddiman, Ronald R. Sederoff, and Vincent L. Chiang. (2011). Membrane protein complexes catalyze both 4- and 3-hydroxylation of cinnamic acid derivatives in monolignol biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 108:21253-21258.

41. Ying-Hsuan Sun, Rui Shi, Xing-Hai Zhang, Vincent L. Chiang, and Ronald R. Sederoff. (2011). MicroRNAs in trees. Plant Mol. Biol. DOI 10.1007/s11103-011-9864-z.

42. Lu, Shanfa, Yang, Chenmin and Chiang, Vincent L. (2011). Conservation and Diversity of MicroRNA-associated Copper-regulatory Networks in Populus trichocarpa. Journal of Integrative Plant Biology 53:879–891.

43. Chuanzhen Zhou, Quanzi Li, Vincent L. Chiang, Lucian A. Lucia, Dieter P. Griffis. (2011). Chemical and Spatial Differentiation of Syringyl and Guaiacyl Lignins in Poplar Wood via Time-of-Flight Secondary Ion Mass Spectrometry. Anal. Chem., 2011:7020-7026.

44. Quanzi Li, Douyong Min, Jack Peng-Yu Wang, Ilona Peszlen, Laszlo Horvath, Balazs Horvath, Yufuko Nishimura, Hasan Jameel, Hou-Min Chang, and Vincent L. Chiang (2011). Down-regulation of glycosyltransferase 8D genes in Populus trichocarpa caused reduced mechanical strength and xylan content in wood. Tree Physiology. 2011:226-236.

45. Ying-Hsuan Sun, Shanfa Lu, Rui Shi, and Vincent L. Chiang (2011). Computational Prediction of Plant miRNA Targets. H. Kodama, A. Komamine (eds.), in RNAi and Plant Gene Function Analysis, Methods in Molecular Biology 744:175-186.

46. Novaes, E., Kirst, M., Chiang, V. Winter-Sederoff, H., and Sederoff, R. (2010). Lignin and Biomass: A Negative Correlation for Wood Formation and Lignin Content in Trees. Plant Physiology 154:555-561.

47. Shi, R., Yang, C., Lu, S., Sederoff, R., and Chiang, V.L. (2010). Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa. Planta 232:1281-1288.

48. Shi, R., Sun, Y-H., Li, Q., Heber, S., Sederoff, R., and Chiang, V.L. (2010). Towards a systems approach for lignin biosynthesis in Populus trichocarpa: Transcript abundance and specificity of the monolignol biosynthetic genes. Plant Cell Physiol. 51:144-163.

49. Sutela, S., Niemi, K., Edesi, J., Laakso, T., Saranpää, P., Vuosku, J., Mäkelä, R., Tiimonen, H., Chiang, V.L., Koskimäki, J., Suorsa, M., Julkunen-Tiittoand R., Häggman, H. (2009). Phenolic compounds in ectomycorrhizal interaction of lignin modified silver birch. BMC Plant Biology 9:124-139.

50. M. Schmidt, A. M. Schwartzberg, P. N. Perera, A. Weber-Bargioni, A. Carroll, P. Sarkar, E. Bosneaga, J. J. Urban, J. Song, M. Y. Balakshin, E. A. Capanema, M. Auer, P. D. Adams, V. L. Chiang, P. James Schuck. (2009). Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa. Planta 230:589-597.

51. Kumar, M., Thammannagowda, S., Bulone, V., Chiang, V., Han, K-H., Joshi, C. P., Mansfield, S. D., Mellerowicz, E., Sundberg, B., Teeri, T., Ellis, B. E. (2009). An update on the nomenclature for the cellulose synthase genes in Populus. Trends in Plant Science 14:248-254.

52. Osakabe, Y., Osakabe, K., and Chiang, V.L. (2009). Characterization of the tissue-specific expression of phenylalanine ammonia-lyase gene promoter from loblolly pine (Pinus taeda) in Nicotiana tabacum. Plant Cell Rep. 28(9):1309-1317.

53. Lu, S., Sun, Y-H., and Chiang, V.L. (2009). Adenylation of plant miRNAs. Nucleic Acids Research. 37:1878-1885.

54. Lu, S., Sun, Y-H., and Chiang, V.L. (2008). Stress-Responsive MicroRNAs in Populus. Plant Journal. 55:131-151.

55. Lu, S., Yi, X., Li., L., Joshi, C.P., and Chiang, V.L. (2008). Differential expression of three eucalyptus secondary cell wall-related cellulose synthase genes in response to tension stress. J. Exp. Bot. 59(3):681-695.

56. Tiimonen, H., Aronen, T., Laakso, T., Saranpää, P., Chiang, V.L., Häggman, H., and Niemi, K. (2008). Paxillus involutus forms an ectomycorrhizal symbiosis and enhances survival of PtCOMT-modified Betula pendula in vitro. Silvae Genetica 57:235-242.

57. Yeh, T.f., Wang, J., Rui, S., Sun, Y.H. and Chiang, V.L. (2007). A Novel O-Methyltransferase-like gene with a drastic ectopic expression in response to tension wood formation in Populus trichocarpa. Cellulose Chem. Technol., 41(9-10):521-528.

58. Nakatsubo, T., Li, L., Hattori, T., Lu, S., Sakakibara, N., Chiang, V.L., Shimada, M., Suzuki, S., and Umezawa, T. (2007). Roles of 5-hydroxyconiferylaldehyde and caffeoyl CoA O-methyltransferases in monolignol biosynthesis in Carthamus tinctorius. Cellulose Chem. Technol., 41(9-10):511-520.

59. Lu, S., Sun, Y.H., Amerson, H., and Chiang, V.L. (2007). MicroRNAs in loblolly pine (Pinus taeda L.) and their association with fusiform rust gall development. Plant Journal. 51: 1077-1098.

60. Hancock, J.E., W.L. Loya, C.P. Giardina, L. Li, V.L. Chiang, K.S. Pregitzer. (2007). Plant Growth, Biomass Partitioning and Soil Carbon Formation in Response to Altered Lignin Biosynthesis in Populus tremuloides. New Phytologist. 173:732–742.

61. Song, J., Lu, S., Chen, Z.Z., Lourenco, R., and Chiang, V.L. (2006). Genetic transformation of Populus trichocarpa genotype Nisqually-1: A functional genomic tool for woody plants. Plant and Cell Physiol. 47:1582-1589.

62. Shiro Suzuki, Laigeng Li, Ying-Hsuan Sun and Vincent L. Chiang. (2006). The cellulose synthase gene superfamily and biochemical functions of xylem-specific cellulose synthase-like genes in Populus trichocarpa. Plant Physiology 142:1233-1245.

63. Shanfa Lu, Yihua Zhou, Laigeng Li, and Vincent L. Chiang. (2006). Distinct Roles of Cinnamate 4-Hydroxylase Genes in Populus. Plant and Cell Physiol. 47:905-914.

64. (Book Chapter) Chen, Z.Z., Ho, C.K., Ahn, I.S., and Chiang, V.L. (2006). Agrobacterium Protocols in “Methods in Molecular Biology, vol. 344”, K. Wang ed., 2^nd ed., Totowa, NJ: Humana Press, vol. 2, pp. 125-134.

65. Li, L., Lu, S., and Chiang, V.L. (2006). A genomic and molecular view of wood formation. Crit. Rev. Plant Sci. 25(3):215-233.

66. Yamada, T., Yeh, T.-F., Chang, H-m., Li, L., Kadla, J.F., and Chiang, V.L. (2006). Rapid analysis of transgenic trees using transmittance near-infrared spectroscopy (NIR). Holzforschung, 60(1):24-28.

67. Lu, S., Sun, Y-H., Shi, R., Clark, C., Li, L., Chiang, V.L. (2005).  Novel and mechanical stress-responsive microRNAs in Populus trichocarpa that are absent from Arabidopsis. The Plant Cell, 17:2186-2203.

68. Tiimonen, H., Aronen, T., Laakso, T., Saranpaa, P., Chiang, V.L., Ylioja, T., Roininen, H. and Haggman, H. (2005). Does lignin modification affect feeding preference or growth performance of insect herbivores in transgenic silver birch (Betula pendula Roth)? Planta 222:699-708.

69. Li, L., Cheng, X., Lu, S., Nakatsubo, T., Umezawa, T., Chiang, V.L. (2005). Clarification of cinnamoyl co-enzyme A reductase catalysis in monolignol biosynthesis of aspen. Plant Cell Physiol. 46:1073-1082.

70. Shi, R. and Chiang, V.L. (2005). Facile means for quantifying microRNA expression by real-time PCR. BioTechniques 39:519-525.

71. Yeh, T.-F., Yamada, T., Capanema, E., Chang, H-m., Chiang, V.L., and Kadla, J.F. (2005). Rapid screening of wood chemical component variations using transmittance near-infrared spectroscopy. J. Agric. Food Chem., 53(9):3328-3332.

72. Lu, S., Shi, R., Tsao, C-C., Yi, X., Li, L., and Chiang, V.L. (2004). RNA silencing in plants by expression of siRNA duplexes. Nucleic Acids Research. 21: e171.

73. Li, L., Zhou, Y., Cheng, X. F., Sun, J., Marita, J., Ralph, J., and Chiang, V.L. (2003). Combinatorial modification of multiple lignin traits in trees through multigene cotransformation. Proceedings of the National Academy of Sciences of the United States of America, 100:4939-4944.

74. Nakatsubo, T., Li, L., Chiang, V. L., Hattori, T., Shimada, M., Umezawa, T. (2003). Basic studies towards elucidation of heartwood formation mechanisms. Wood Research 90:5-6.

75. Chiang, V.L. (2002). From rags to riches. Nature Biotechnology 20:557-558.

76. Li, L., Cheng, X.F., Leshkevich, J., Umezawa, T., Suzuki, S., Harding, S.A., and Chiang, V.L. (2001). The last step of syringyl monolignol biosynthesis in angiosperm dicots is regulated by a novel gene encoding sinapyl alcohol dehydrogenase. Plant Cell 13:1567-1585.

77. Harding, S.A., Leshkevich, J., Chiang, V.L. and Tsai, C.J. (2002). Differential substrate inhibition couples kinetically distinct 4-coumarate; CoA ligase with spatially distinct structural and metabolic roles in quaking aspen. Plant Physiology, 128:428-438.

78. Wu, L., Joshi, C.P. and Chiang, V.L. (2000). A xylem-specific cellulose synthase gene from aspen is responsive to tension stress.  Plant Journal. 22:495 502.

79. Li, L., Popko, J.L., Umezawa, T. and Chiang, V.L. (2000). 5-Hydroxyconiferyl aldehyde modulates enzymatic methylation for syringyl monolignol formation, a new view of monolignol biosynthesis in angiosperms. J. Biol. Chem. 275:6537-6545.

80. Hu, W.-J., Lung, J., Harding, S.A., Popko, J.L., Ralph, J., Stokke, D.D., Tsai, C.-J., and    Chiang, V.L. (1999). Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees. Nature Biotechnology 17:808-812.

81. Osakabe, K., Tsao, C.C., Li, L., Popko, J.L., Umezawa, T., Carraway, D.T., Smeltzer, R.H., Joshi, C.P. and Chiang, V.L. (1999). Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms. Proceedings of the National Academy of Sciences of the United States of America 96:8955-8960.

82. Li, L., Osakabe, Y., Joshi, C.P., Chiang, V.L. (1999). Secondary xylem-specific expression of caffeoyl-coenzyme A 3-O-methyltransferase plays an important role in the methylation pathway associated with lignin biosynthesis in loblolly pine. Plant Molecular Biology 40:555-565.

83. Hu, W.-J., Kawaoka, A., Tsai, C.-J., Lung, J., Osakabe, K., Ebinuma, H., and Chiang, V.L. (1998).  Compartmentalized expression of two structurally and functionally distinct 4-coumarate: coenzyme A ligase (4CL) genes in Aspen (Populus tremuloides).  Proceedings of the National Academy of Sciences of the United States of America 95:5407-5412.

84. Wu, L., Joshi, C.P., and Chiang, V.L. (1998). AraxCelA, A New Member of Cellulose Synthase Gene family from Arabidopsis thaliana. Plant Physiology, 117:1125.

85. Ho, C.-K., S.-H. Chang, J.-Y. Tsai, C.-J. Tsai, V.L. Chiang and Z.-Z. Chen. (1998). Agrobacterium tumefaciens-mediated transformation of Eucalyptus camaldulensis and production of transgenic plants.  Plant Cell Reports 17:675-680.

86. Tsai, C.-J., Popko, J.L., Mielke, M.R., Hu, W.-J., Podila, G.K., and Chiang, V.L. (1998). Suppression of O-methyltransferase gene by homologous sense transgene in quaking aspen causes red-brown wood phenotypes.  Plant Physiology, 117:101-112.

87. Joshi, C.P., and Chiang, V.L. (1998).  Conserved Sequence Motifs in Plant S-Adenosyl-L-Methionine-Dependent Methyltransferases.  Plant Molecular Biology 37:663-674.

88. Li, L., Zhang, X.-H., Joshi, C.P., and Chiang, V.L. (1998). Compression Stress Responsive Expression of Ferritin and Peroxidase Genes in the Developing Xylem of Loblolly Pine (Pinus taeda). Plant Physiology, 116:1604.

89. (Book Chapter) Karnosky, D., Shin, D., Podila, G.K., Chiang, V.L. and Riemenschneider, D.E. (1998). Biotechnology in Forest Tree Improvement, In: Forest Genetics and Tree Breeding.  A.K. Mandal and G.L. Gibson Editors.  CBS Publishers & Distributors, New Delhi, India. 52-268.

90. Hu, W.-J., Kawaoka, A., Osakabe, K., Tsai, C.-J., Popko, J.L. and Chiang, V.L. (1997). p coumaric acid: CoA ligase genes and their roles in the biosynthesis of monolignols in quaking aspen (Populus tremuloides Michx.). Plant Physiology, 114(3):179-180.

91. Tsai, C.-J., Mielke, M.R., Popko, J.L., Podila, G.K., and Chiang, V.L. (1997). Novel wood coloration and altered lignin composition in transgenic aspen through manipulation of caffeic acid/5 hydroxyferulic acid O methyltransferase gene expression. Plant Physiology, 114(3):300.

92. Joshi, C.P., Zhou, H., Huang, X., and Chiang, V.L. (1997).  Context sequences of translation initiation codon in plants.  Plant Molecular Biology 35:993-1001

93. Li, L., Popko, J.L., Zhang, X-H., Osakabe, K., Tsai, C.-J., Joshi, C.P. and V.L. Chiang.  (1997). A novel multifunctional O-methyltransferase implicated in a dual methylation pathway associated with lignin biosynthesis in loblolly pine. Proceedings of the National Academy of Sciences of the United States of America 94:5431-5466.  

94. (Book Chapter) Boerjan, W., M. Baucher, B. Chabbert, M. Petit-Conil, J.C. Leple, G. Pilate, D. Cornu, B. Monties, M. Van Montagu, J. Van Doorsselaere, D. Inze, L. Jouanin, C.-J. Tsai, G.K. Podila, C.P. Joshi and V.L. Chiang. (1997). Genetic modification of lignin biosynthesis in quaking aspen (Populus tremuloides x Populus alba).  In: Populus: A model system for tissue culture and molecular biology of woody plants.  N.B. Klopfenstein, Eds. Micropropagation, Genetic Engineering, and Molecular Biology of Populus, United States Department of Agriculture Forest Service, Rocky Mountain Forest and Range Experiment Station, Ft. Collins, CO. 193-205.

95. Zhang, X.-H., and V.L. Chiang. (1997). Molecular cloning of a 4-coumarate: coenzyme A ligase (4CL) in loblolly pine and the roles of this enzyme in the biosynthesis of lignin in compression wood.  Plant Physiology 113:65-74.

96. (Book Chapter) C.P. Joshi, Kumar S., Klueva, N, Joshi R., Nguyen H.T., Hao L., Zhang X, Tsao C. and Chiang V. (1996). Modified Differential display for the cloning of multigene family members in plants. Invited talk at “Differential display and related techniques for gene discovery” meeting, Cold Spring Harbor Laboratory, New York, USA, 1996.

97. Tsai, C.-J., G.K. Podila and V.L. Chiang. (1996). 3' cycle-labeled oligonucleotides with predictable length for primer extension and transgenic analysis.  Nucleic Acids Research 24:5060-5062.

98. Ge, L. and V.L. Chiang. (1996). A full-length cDNA encoding trans-cinnamate 4-hydroxylase from developing xylem of Populus tremuloides.  Plant Gene Register 96-075.

99. Zhang, X.-H. and V.L. Chiang. (1996). Single-stranded DNA ligation by T4 RNA ligase for PCR cloning of 5'-non-coding fragments and coding sequence of a specific gene.  Nucleic Acids Research 24:990-991.