报告题目一：The opium poppy genome and morphinan production
报 告 人一：叶凯
报告题目二：Friend or Foe: Dissecting molecular mechanisms underlying Arabidopsis interactions with Fusarium oxysporum using dual RNA-seq
报 告 人二：郭立
主办单位：林木遗传育种国家重点实验室
时 间：2019年05月27日（星期一） 9:00
地 点：科技楼主310
报告人简介：
叶凯，西安交通大学教授。2008年在荷兰莱顿大学获得博士学位；2008-2009在欧洲生物信息学研究所从事博士后研究；2009-2012在莱顿大学担任助理教授；2012-2016在美国圣路易斯华盛顿大学担任助理教授；2016-02-至今，西安交通大学教授，博士生导师，西安交通大学青年拔尖人才计划，“叶凯青年科学家工作室”负责人。叶凯教授近10年来一直专注基因组中结构变异的格局、机制及其功能进化，得到了国内外同行的高度认可与重视，受邀加入国际千人基因组计划（1000 Genomes Project）、国际肿瘤基因组计划（TCGA）、国际基因组结构变异计划（HGSVC）等多个国际旗舰基因组计划。多次作为Invited speaker在业界影响力重大国际会议上作学术报告。在其51篇SCI论文中，有25篇为近五年发表，以主要作者（第一作者、共同第一作者、通讯作者、共同通讯作者）发表论文11篇。刊载近五年5篇代表性论文的期刊平均影响因子27.542（Science、Nature、Nature Medicine、Nature Communications、Genome Research）。论文SCI他引总数为15,918，近五年SCI他引总数为13,038；单篇最高SCI他引为3,399，单篇SCI他引过百次文章14篇，12篇入选ESI高被引论文。
郭立，西安交通大学副教授。2012年在美国宾夕法尼亚州立大学植物病理系获得博士学位；2012-2016年在美国马萨诸塞州大学从事植物病原基因组学和生物信息学方向的博士后研究。2016年10月进入西安交通大学电子与信息工程学院从事教学科研工作，研究方向包括植物基因组学、植物病原互作机制和基因调控网络。现主持国家自然科学基金青年基金、国家重点研发项目子课题，并参与国家自然科学基金面上项目1项。现已在Science, Nature Communications, New Phytologist, Molecular Plant Pathology, Frontiers in Plant Science, IEEE Transactions on Computational Biology and Bioinformatics等多个国际权威期刊发表高水平SCI论文16篇，其中第一作者发表SCI论文7篇，参与编写专著2部，为多个国际权威期刊担任同行审稿人20余次，参加国际国内学术会议16次，做特邀报告5次，担任国际研讨会Workshop特邀讲员2次。
报告内容简介：
报告一简介：Morphinan-based painkillers are derived from opium poppy (Papaver somniferum L.). We report a draft of the opium poppy genome, with 2.72 gigabases assembled into 11 chromosomes with contig N50 and scaffold N50 of 1.77 and 204 megabases, respectively. Synteny analysis suggests a whole-genome duplication at ~7.8 million years ago and ancient segmental or whole-genome duplication(s) that occurred before the Papaveraceae-Ranunculaceae divergence 110 million years ago. Syntenic blocks representative of phthalideisoquinoline and morphinan components of a benzylisoquinoline alkaloid cluster of 15 genes provide insight into how this cluster evolved. Paralog analysis identified P450 and oxidoreductase genes that combined to form the STORR gene fusion essential for morphinan biosynthesis in opium poppy. Thus, gene duplication, rearrangement, and fusion events have led to evolution of specialized metabolic products in opium poppy.
报告二简介：Fusarium oxysporum are filamentous ascomycetes causing vascular wilt diseases to over 100 species of plants, but many isolates colonize plants as endophytes or symbionts. We observe that Arabidopsis thaliana Col-0 plants infected by a pathogenic (Fo5176) and biocontrol (Fo47) strain display distinct phenotypes in symptoms, growth and development of roots and water contents. How plants respond to the pathogenic vs symbiotic F. oxysporum is unknown. To dissect such mechanisms, we performed time-series dual RNA-seq to compare the two distinct interactions. Root infection transcriptome comparison at 12, 24, 48 and 96 hpi (hours post inoculation) shows that A. thaliana undergoes distinct transcriptomic changes against Fo5176 and Fo47 infection. Clustering analysis identifies two gene co-expression clusters that are highly enriched in plant defense and immune response, where one (PTI response) is induced in both Fo5176 and Fo47, but the other (ETI response) has a bipolar induction pattern. Furthermore, Fo47 other than Fo5176 infected plants show induced genes enriched in nutrient assimilation, plant growth and development. Pathway enrichment analysis suggests auxin-response, nitrate and potassium transport pathways are upregulated in Fo47-infected plants compared to Fo5176 and control. Transcription factor gene family analysis suggests several families such as Nin-like proteins (NLPs) are upregulated in Fo47-infected plants. Our results indicate A. thaliana forms a symbiotic relationship with Fo47, and the symbiont promotes host growth and development likely through specific activation of host gene modules contributing to host enhanced nutrient assimilation.
欢迎各位老师、同学参与交流！
林木遗传育种国家重点实验室
2019年05月20日