姜立泉教授

 

中文名字 姜立泉        
英文名字 Vincent L. Chiang
美国北卡罗莱纳州立大学杰出教授
美国北卡罗莱纳州立大学,自然资源学院,林业与环境资源系,
林业生物技术团队,农业暨生命科学学院, 植物暨微生物系。
校园邮箱 7247
美国北卡,罗利,27695-7247, 电话:919/513-0098
电子邮箱:vchiang@ncsu.edu
 
学历
理学学士,1976,中国文化大学,台湾 (化学);
理学硕士,1980,华盛顿大学(化学)
    士,1983,华盛顿大学(化学和生物化学)
 
工作经历
2008至今北卡罗莱纳州立大学杰出教授(自然资源创新)
2013至今东北林业大学林木遗传育种国家重点实验室客座教授
2002至今北卡罗莱纳州立大学林业生物技术团队教授,主任
2004至今北卡罗莱纳州立大学林业生物技术工业研究协会主任
2007至今台湾台北中央研究院农业生物技术研究中心,学术顾问委员会成员
2011-至今台湾台北,国立台湾大学客座教授
2006-2009 北卡罗莱纳州罗利,林业生物技术研究所,董事会成员
2001春季日本京都,京都大学,日本科学促进会,杰出访问教授
1995-2002 密歇根理工大学植物生物技术研究中心主任
1985-1992 密歇根理工大学林学院助理教授,副教授,教授
1987-2002 密歇根理工大学生物科学系副教授,教授
1983-1985 华盛顿,西雅图,华盛顿大学助理研究员
 
荣誉和奖励
美国科学促进会会士
国际木材科学研究院院士
北卡罗莱纳州立大学杰出教授研究奖
密歇根理工大学特聘教授研究奖
密歇根州立大学理事会杰出教员奖
美国国家自然科学基金生物技术燃料项目小组成员
美国能源部生物能源科学项目小组成员
美国农业部国家研究项目竞争基金小组成员
 
领导岗位工作概述
1.具有在研究中心超过15年的管理工作经验,领导研究中心员工、博士后、研究生开展生物化学、分子生物学和生物技术等多学科、 跨学科的研究工作。
2.1995年,在密歇根理工大学建立了第一个生物技术研究中心—— 植物生物技术研究中心 (PBRC)。
3.1995年至2002年,在密歇根理工大学作为PBRC的主任,管理超 过20名研究人员,其中包括3名终身制人员。
4.管理研究中心的研究经费,年度研究经费大约为100万美元。
5.自2002年起,作为北卡罗莱纳州立大学林业生物技术研究组的联合主任,管理超过20名研究和行政人员。
6.自2004年起,建立了北卡罗莱纳州立大学林业生物技术工业研究协会 (FORBIRC),招募了全球13个生物技术公司和15名专职人 员。
7.管理FORBIRC所有的研究活动,并通过协会会费建立了竞争性基金资源,以资助协会会员每年4-5个研究项目。
8.自2002年起,管理林业生物技术组的研究经费,年度研究经费大 约为200万美元。
9.组织和管理美国国家自然科学基金会和美国能源部资助的高达数百万美元的植物基因组研究计划,该计划共有37名研究人员参加。
 
主要研究成果
I. 概述:
获得的总研究经费:30190168美元(约3000万美元)
直接指导博士后总人数:30人
直接指导的高级研究人员总人数:8人
直接指导的研究生总人数:博士12人;硕士15人
直接指导的访问教授/学者总人数:12人
直接指导的实验室管理人员和技术人员总人数:6人
学术报告和主题演讲总数:25次(自2002年起)
美国和欧洲专利总数:21个
II.发表论文及著作 :
1.Ying-Chung Lin, Wei Li, Hao Chen, Quanzi Li, Ying-Hsuan Sun, Rui Shi, Chien-Yuan Lin, Jack P. Wang, Hsi-Chuan Chen, Ling Chuang, Guan-Zheng Qu, Ronald R. Sederoff, and Vincent L. Chiang (2014). A simple high throughput xylem protoplast system for studying wood formation. Nat. Protocols (Invited submission)
2.Wei Li, Ying-Chung Lin, Quanzi Li, Rui Shi, Chien-Yuan Lin, Hao Chen, Ling Chuang, Guan-Zheng Qu, Ronald R. Sederoff, and Vincent L. Chiang (2014). A robust anti-transcription factor (TF) antibody-based chromatin immunoprecipitation (ChIP) protocol for studying TF direct targets in woody plants. Nat. Protocols (Invited submission)
3.Jack P. Wang, Punith P. Naik, Hsi-Chuan Chen, Rui Shi, Chien-Yuan Lin, Jie Liu, Christopher M. Shuford, Quanzi Li, Cranos M. Williams, David C. Muddiman, Joel J. Ducoste, Ronald R. Sederoff, and Vincent L. Chiang. (2014). Complete proteomic based enzyme reaction and inhibition kinetics reveal how monolignol biosynthetic enzyme families affect metabolic-flux and lignin. Plant Cell. 10.1105/tpc.113.120881. (In press)
4.Hsi-Chuan Chen, Jina Song, Jack P. Wang, Ying-Chung Lin, Joel Ducoste, Christopher M. Shuford, Jie Liu, Quanzi Li, Rui Shi, Angelito Nepomuceno, Fikret Isik, David C. Muddiman, Cranos Williams, Ronald R. Sederoff, and Vincent L. Chiang. (2014). Systems biology of lignin biosynthesis in Populus trichocarpa: Heteromeric 4-coumaric acid: CoA ligase (4CL) protein complex formation, regulation and numerical modeling. Plant Cell. 10.1105/tpc.113.119685.(In press)
5.Amborella Genome Project. (2013). The Amborella Genome and the Evolution of Flowering Plants. Science 342: 1241089-10.
6.Lin, Y-C., Li, W., Sun, Y-H., Kumari, S., Wei, H., Li, Q., Tunlaya-Anukit, S., Sederoff, R.R. and Chiang, V.L. (2013). SND1 transcription factor-directed quantitative functional hierarchical genetic regulatory network in wood formation in Populus trichocarpa. Plant Cell. 25: 4321-4341.
7.Philip Loziuk, Jack Wang, Quanzi Li, Ronnald Sederoff, Vincent Chiang, and David Muddiman. (2013). Understanding the role of proteolytic digestion on discovery and targeted proteomic measurements using liquid chromatography tandem mass spectrometry and design of experiments. Journal of Proteome Research.DOI: 10.1021/pr4008442.
8.Shanfa Lu, Quanzi Li, Hairong Wei, Mao-Ju Chang, Sermsawat Tunlaya-Anukit, Hoon Kim, Jie Liu, Jingyuan Song, Ying-Hsuan Sun, Lichai Yuan, Ting-Feng Yeh, John Ralph, Ronald R. Sederoff, and Vincent L. Chiang. (2013). Ptr-miR397a is a master negative regulator of laccase genes affecting lignin polymerization in Populus trichocarpa. Proceedings of the National Academy of Sciences, USA. 110: 10848-10853.
9.Hsi-Chuan Chen, Jina Song, Cranos M. Williams, Christopher M. Shuford, Jie Liu, Jack P. Wang, Quanzi Li, Rui Shi, Emine Gokce, Joel Ducoste, David C. Muddiman, Ronald R. Sederoff, and Vincent L. Chiang. (2013). Monolignol Pathway 4-Coumaric Acid:Coenzyme A Ligases in Populus. trichocarpa: Novel Specificity, Metabolic Regulation, and Simulation of Coenzyme A Ligation Fluxes. Plant Physiol. 161:1501-1516.
10.Shi R, Shuford CM, Wang JP, Sun YH, Yang Z, Chen HC, Tunlaya-Anukit S, Li Q, Liu J, Muddiman DC, Sederoff RR, Chiang VL. (2013). Regulation of phenylalanine ammonia-lyase (PAL) gene family in wood forming tissue of Populus trichocarpa. Planta 238:487-97.
11.Quanzi Li, Ying-Chung Lin, Ying-Hsuan Sun, Jian Song, Hao Chen, Xing-Hai Zhang, Ronald R. Sederoff, and Vincent L. Chiang. (2012). Splice variant of the SND1 transcription factor is a dominant negative of SND1 members and their regulation in Populus trichocarpa. Proceedings of the National Academy of Sciences, USA. 109: 14699-14704.
12.P. N. Perera, M. Schmidt, V. L. Chiang, P. J. Schuck, and P. D. Adams. (2012) Raman-spectroscopy-based noninvasive microanalysis of native lignin structure. Anal. Bioanal. Chem. 402:983-987.
13.Jie Liu, Rui Shi, Quanzi Li, Ronald R. Sederoff, Vincent L. Chiang. (2012). A standard reaction condition and a single HPLC separation system are sufficient for estimation of monolignol biosynthetic pathway enzyme activities. Planta 236:879-885.
14.Shuford CM, Sederoff RR, Chiang VL, Muddiman DC. (2012). Peptide production and decay rates affect the quantitative accuracy of protein cleavage isotope dilution mass spectrometry (PC-IDMS). Mol Cell Proteomics 11:814-823.
15.Shuford, Christopher; Li, Quanzi; Sun, Ying-Hsuan; Chen, Hsi-Chuan; Wang, Jack; Shi, Rui; Sederoff, Ronnald; Chiang, Vincent; Muddiman, David. (2012). Comprehensive Quantification of Monolignol-pathway Enzymes in Populus trichocarpa by Protein Cleavage Isotope Dilution Mass Spectrometry. Journal of Proteome Research 11:3390-3404.
16.Jack P. Wang, Christopher M. Shuford, Quanzi Li, Jina Song, Ying-Chung Lin, Ying-Hsuan Sun, Hsi-Chuan Chen, Cranos M. Williams, David C. Muddiman, Ronald R. Sederoff, Vincent L. Chiang. (2012). Functional Redundancy of the Two 5-Hydroxylases in Monolignol Biosynthesis of Populus trichocarpa: LC-MS/MS Based Protein Quantification and Metabolic Flux Analysis. Planta 236:795-808.
17.Hsi-Chuan Chen, Quanzi Li, Christopher M. Shuford, Jie Liu, David C. Muddiman, Ronald R. Sederoff, and Vincent L. Chiang. (2011). Membrane protein complexes catalyze both 4- and 3-hydroxylation of cinnamic acid derivatives in monolignol biosynthesis. Proceedings of the National Academy of Sciences, USA108:21253-21258.
18.Sun, Y.H., Shi, R., Zhang, X-H., Chiang, V.L., Sederoff, R. (2011). MicroRNAs in trees. Plant Mol. Biol. DOI 10.1007/s11103-011-9864-z.
19.Lu, Shanfa, Yang, Chenmin and Chiang, Vincent L. (2011). Conservation and Diversity of MicroRNA-associated Copper-regulatory Networks in Populus trichocarpa. Journal of Integrative Plant Biology 53:879–891.
20.Zhou, Chuanzhen; Li,Quanzi; Chiang,Vincent L.; Lucia,Lucian A.; Griffis,Dieter P. (2011). Chemical and Spatial Differentiation of Syringyl and Guaiacyl Lignins in Poplar Wood via Time-of-Flight Secondary Ion Mass Spectrometry. Anal. Chem., 2011:7020-7026.
21.Quanzi Li, Douyong Min, Jack Peng-Yu Wang, Ilona Peszlen, Laszlo Horvath, Balazs Horvath, Yufuko Nishimura, Hasan Jameel, Hou-Min Chang, and Vincent L. Chiang (2011). Down-regulation of glycosyltransferase 8D genes in Populus trichocarpa caused reduced mechanical strength and xylan content in wood. Tree Physiol. 2011:226-236.
22.Ying-Hsuan Sun, Shanfa Lu, Rui Shi, and Vincent L. Chiang (2011). Computational Prediction of Plant miRNA Targets. H. Kodama, A. Komamine (eds.), in RNAi and Plant Gene Function Analysis, Methods in Molecular Biology 744:175-186.
23.Novaes, E., Kirst, M., Chiang, V. Winter-Sederoff, H., and Sederoff, R. (2010). Lignin and Biomass: A Negative Correlation for Wood Formation and Lignin Content in Trees. Plant Physiol. 154:555-561.
24.Shi, R., Yang, C., Lu, S., Sederoff, R., and Chiang, V.L. (2010). Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa. Planta 232:1281-1288.
25.Shi, R., Sun, Y-H., Li, Q., Heber, S., Sederoff, R., and Chiang, V.L. (2010). Towards a systems approach for lignin biosynthesis in Populus trichocarpa: Transcript abundance and specificity of the monolignol biosynthetic genes. Plant Cell Physiol. 51:144-163.
26.Sutela, S., Niemi, K., Edesi, J., Laakso, T., Saranpää, P., Vuosku, J., Mäkelä, R., Tiimonen, H., Chiang, V.L., Koskimäki, J., Suorsa, M., Julkunen-Tiitto and R., Häggman, H. (2009). Phenolic compounds in ectomycorrhizal interaction of lignin modified silver birch. BMC Plant Biology 9:124-139.
27.M. Schmidt, A. M. Schwartzberg, P. N. Perera, A. Weber-Bargioni, A. Carroll, P. Sarkar, E. Bosneaga, J. J. Urban, J. Song, M. Y. Balakshin, E. A. Capanema, M. Auer, P. D. Adams, V. L. Chiang, P. James Schuck. (2009). Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa. Planta 230:589-597.
28.Kumar, M., Thammannagowda, S., Bulone, V., Chiang, V., Han, K-H., Joshi, C. P., Mansfield, S. D., Mellerowicz, E., Sundberg, B., Teeri, T., Ellis, B. E. (2009). An update on the nomenclature for the cellulose synthase genes in Populus. Trends in Plant Science 14:248-254.
29.Osakabe, Y., Osakabe, K., and Chiang, V.L. (2009). Characterization of the tissue-specific expression of phenylalanine ammonia-lyase gene promoter from loblolly pine (Pinus taeda) in Nicotiana tabacum. Plant Cell Rep. 28(9):1309-1317.
30.Lu, S., Sun, Y-H., and Chiang, V.L. (2009). Adenylation of plant miRNAs. Nucleic Acids Research. 37:1878-1885.
31.Lu, S., Sun, Y-H., and Chiang, V.L. (2008). Stress-Responsive MicroRNAs in Populus. Plant J. 55:131-151.
32.Lu, S., Yi, X., Li., L., Joshi, C.P., and Chiang, V.L. (2008). Differential expression of three eucalyptus secondary cell wall-related cellulose synthase genes in response to tension stress.J. Exp. Bot.59(3):681-695.
33.Tiimonen, H., Aronen, T., Laakso, T., Saranpää, P., Chiang, V.L., Häggman, H., and Niemi, K. (2008). Paxillus involutus forms an ectomycorrhizal symbiosis and enhances survival of PtCOMT-modified Betula pendula in vitro. Silvae Genetica 57:235-242.
34.Yeh, T.f., Wang, J., Rui, S., Sun, Y.H. and Chiang, V.L. (2007). A Novel O-Methyltransferase-like gene with a drastic extopic expression in response to tension wood formation in Poupulus trichocarpa. Cellulose Chem. Technol., 41(9-10):521-528.
35.Nakatsubo, T., Li, L., Hattori, T., Lu, S., Sakakibara, N., Chiang, V.L., Shimada, M., Suzuki, S., and Umezawa, T. (2007). Roles of 5-hydroxyconiferylaldehyde and caffeoyl CoA O-methyltransferases in monolignol biosynthesis in Carthamus tinctorius. Cellulose Chem. Technol., 41(9-10):511-520.
36.Lu, S., Sun, Y.H., Amerson, H., and Chiang, V.L. (2007). MicroRNAs in loblolly pine (Pinus taeda L.) and their association with fusiform rust gall development. Plant J. 51: 1077-1098.
37.Hancock, J.E., W.L. Loya, C.P. Giardina, L. Li, V.L. Chiang, K.S. Pregitzer. (2007). Plant Growth, Biomass Partitioning and Soil Carbon Formation in Response to Altered Lignin Biosynthesis in Populus tremuloides.New Phytologist. 173:732–742.
38.Song, J., Lu, S., Chen, Z.Z., Lourenco, R., and Chiang, V.L. (2006). Genetic transformation of Populus trichocarpa genotype Nisqually-1: A functional genomic tool for woody plants. Plant and Cell Physiol. 47:1582-1589.
39.Shiro Suzuki, Laigeng Li, Ying-Hsuan Sun and Vincent L. Chiang. (2006). The cellulose synthase gene superfamily and biochemical functions of xylem-specific cellulose synthase-like genes in Populus trichocarpa. Plant Physiol. 142:1233-1245.
40.Shanfa Lu, Yihua Zhou, Laigeng Li, and Vincent L. Chiang. (2006). Distinct Roles of Cinnamate 4-Hydroxylase Genes in Populus. Plant and Cell Physiol. 47:905-914.
41.(Book Chapter) Chen, Z.Z., Ho, C.K., Ahn, I.S., and Chiang, V.L. (2006). Agrobacterium Protocols in “Methods in Molecular Biology, vol. 344”, K. Wang ed., 2nd ed., Totowa, NJ: Humana Press, vol. 2, pp. 125-134.
42.Li, L., Lu, S., and Chiang, V.L. (2006). A genomic and molecular view of wood formation. Crit. Rev. Plant Sci. 25(3):215-233.
43.Yamada, T., Yeh, T.-F., Chang, H-m., Li, L., Kadla, J.F., and Chiang, V.L. (2006). Rapid analysis of transgenic trees using transmittance near infrared spectroscopy (NIR). Holzforschung, 60(1):24-28.
44.Lu, S., Sun, Y-H., Shi, R., Clark, C., Li, L., Chiang, V.L. (2005). Novel and mechanical stress-responsive microRNAs in Populus trichocarpa that are absent from Arabidopsis. The Plant Cell, 17:2186-2203.
45.Tiimonen, H., Aronen, T., Laakso, T., Saranpaa, P., Chiang, V.L., Ylioja, T., Roininen, H. and Haggman, H. (2005). Does lignin modification affect feeding preference or growth performance of insect herbivores in transgenic silver birch (Betula pendula Roth)? Planta 222:699-708.
46.Li, L., Cheng, X., Lu, S., Nakatsubo, T., Umezawa, T., Chiang, V.L. (2005). Clarification of cinnamoyl co-enzyme A reductase catalysis in monolignol biosynthesis of aspen. Plant Cell Physiol. 46:1073-1082.
47.Shi, R. and Chiang, V.L. (2005). Facile means for quantifying microRNA expression by real-time PCR. BioTechniques 39:519-525.
48.Yeh, T.-F.,Yamada, T., Capanema, E., Chang, H-m., Chiang, V.L., and Kadla, J.F. (2005). Rapid screening of wood chemical component variations using transmittance near-infrared spectroscopy. J. Agric. Food Chem., 53(9):3328-3332.
49.Lu, S., Shi, R., Tsao, C-C., Yi, X., Li, L., and Chiang, V.L. (2004). RNA silencing in plants by expression of siRNA duplexes. Nucleic Acids Res. 21: e171.
50.Li, L., Zhou, Y., Cheng, X. F., Sun, J., Marita, J., Ralph, J., and Chiang, V.L. (2003). Combinatorial modification of multiple lignin traits in trees through multigene cotransformation. Proc. Natl. Acad. Sci. USA., 100:4939-4944.
51.Nakatsubo, T., Li, L., Chiang, V. L., Hattori, T., Shimada, M., Umezawa, T. (2003). Basic studies towards elucidation of heartwood formation mechanisms. Wood Research 90:5-6.
52.Chiang, V.L. (2002). From rags to riches. Nature Biotechnology 20:557-558.
53.Li, L., Cheng, X.F., Leshkevich, J., Umezawa, T., Suzuki, S., Harding, S.A., and Chiang, V.L. (2001). The last step of syringyl monolignol biosynthesis in angiosperm dicots is regulated by a novel gene encoding sinapyl alcohol dehydrogenase. Plant Cell 13:1567-1585.
54.Harding, S.A., Leshkevich, J., Chiang, V.L. and Tsai, C.J. (2002). Differential substrate inhibition couples kinetically distinct 4-coumarate; CoA ligase with spatially distinct structural and metabolic roles in quaking aspen. Plant Physiol.,128:428-438.
55.Wu, L., Joshi, C.P. and Chiang, V.L. (2000). A xylem-specific cellulose synthase gene from aspen is responsive to tension stress. Pant J. 22:495‑502.
56.Li, L., Popko, J.L., Umezawa, T. and Chiang, V.L. (2000). 5-Hydroxyconiferyl aldehyde modulates enzymatic methylation for syringyl monolignol formation, a new view of monolignol biosynthesis in angiosperms. J. Biol. Chem. 275:6537-6545.
57.Hu, W.-J., Lung, J., Harding, S.A., Popko, J.L., Ralph, J., Stokke, D.D., Tsai, C.-J., and    Chiang, V.L. (1999). Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees. Nature Biotechnology 17:808-812.
58.Osakabe, K., Tsao, C.C., Li, L., Popko, J.L., Umezawa, T., Carraway, D.T., Smeltzer, R.H., Joshi, C.P. and Chiang, V.L. (1999). Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms. Proceedings of the National Academy of Sciences, USA 96:8955-8960.
59.Li, L., Osakabe, Y., Joshi, C.P., Chiang, V.L. (1999). Secondary xylem-specific expression of caffeoyl-coenzyme A 3-O-methyltransferase plays an important role in the methylation pathway associated with lignin biosynthesis in loblolly pine. Plant Molecular Biology 40:555-565.
60.Hu, W.-J., Kawaoka, A., Tsai, C.-J., Lung, J., Osakabe, K., Ebinuma, H., and Chiang, V.L. (1998). Compartmentalized expression of two structurally and functionally distinct 4-coumarate: coenzyme A ligase (4CL) genes in Aspen (Populus tremuloides). Proceedings of the National Academy of Sciences, USA 95:5407-5412.
61.Wu, L., Joshi, C.P., and Chiang, V.L. (1998). AraxCelA, A New Member of Cellulose Synthase Gene family from
Arabidopsis thaliana. Plant Physiol., 117:1125.

62.Ho, C.-K., S.-H. Chang, J.-Y. Tsai, C.-J. Tsai, V.L. Chiang and Z.-Z. Chen. (1998). Agrobacterium tumefaciens-mediated transformation of Eucalyptus camaldulensis and production of transgenic plants. Plant Cell Reports 17:675-680.
63.Tsai, C.-J., Popko, J.L., Mielke, M.R., Hu, W.-J., Podila, G.K., and Chiang, V.L. (1998). Suppression of O-methyltransferase gene by homologous sense transgene in quaking aspen causes red-brown wood phenotypes. Plant Physiol., 117:101-112.
64.Joshi, C.P., and Chiang, V.L. (1998). Conserved Sequence Motifs in Plant S-Adenosyl-L-Methionine-Dependent Methyltransferases. Plant Molecular Biology 37:663-674.
65.Li, L., Zhang, X.-H., Joshi, C.P., and Chiang, V.L. (1998). Compression Stress Responsive Expression of Ferritin and Peroxidase Genes in the Developing Xylem of Loblolly Pine (Pinus taeda). Plant Physiol., 116:1604.
66.(Book Chapter) Karnosky, D., Shin, D., Podila, G.K., Chiang, V.L. and Riemenschneider, D.E. (1998). Biotechnology in Forest Tree Improvement, In: Forest Genetics and Tree Breeding. A.K. Mandal and G.L. Gibson Editors. CBS Publishers & Distributors, New Delhi, India. 52-268.
67.Hu, W.-J., Kawaoka, A., Osakabe, K., Tsai, C.-J., Popko, J.L. and Chiang, V.L. (1997). p‑coumaric acid: CoA ligase genes and their roles in the biosynthesis of monolignols in quaking aspen (populus tremuloides Michx.). Plant Physiol., 114(3):179-180.
68.Tsai, C.-J., Mielke, M.R., Popko, J.L., Podila, G.K., and Chiang, V.L. (1997). Novel wood coloration and altered lignin composition in transgenic aspen through manipulation of caffeic acid/5‑hydroxyferulic acid O‑methyltransferase gene expression. Plant Physiol., 114(3):300.
69.Joshi, C.P., Zhou, H., Huang, X., and Chiang, V.L. (1997). Context sequences of translation initiation codon in plants. Plant Molecular Biology 35:993-1001
70.Li, L., Popko, J.L., Zhang, X-H., Osakabe, K., Tsai, C.-J., Joshi, C.P. and V.L. Chiang. (1997). A novel multifunctional O-methyltransferase implicated in a dual methylation pathway assoicated with lignin biosynthesis in loblolly pine. Proceedings of the National Academy of Sciences, USA 94:5431-5466. 
71.(Book Chapter) Boerjan, W., M. Baucher, B. Chabbert, M. Petit-Conil, J.C. Leple, G. Pilate, D. Cornu, B. Monties, M. Van Montagu, J. Van Doorsselaere, D. Inze, L. Jouanin, C.-J. Tsai, G.K. Podila, C.P. Joshi and V.L. Chiang. (1997). Genetic modification of lignin biosynthesis in quaking aspen (Populus tremuloides x Populus alba). In: Populus: A model system for tissue culture and molecular biology of woody plants. N.B. Klopfenstein, Eds. Micropropagation, Genetic Engineering, and Molecular Biology of Populus, United States Department of Agriculture Forest Service, Rocky Mountain Forest and Range Experiment Station, Ft. Collins, CO. 193-205.
72.Zhang, X.-H., and V.L. Chiang. (1997). Molecular cloning of a 4-coumarate: coenzyme A ligase (4CL) in loblolly pine and the roles of this enzyme in the biosynthesis of lignin in compression wood. Plant Physiology 113:65-74.
73.(Book Chapter) C.P. Joshi, Kumar S., Klueva, N, Joshi R., Nguyen H.T., Hao L., Zhang X, Tsao C. and Chiang V. (1996). Modified Differential display for the cloning of multigene family members in plants. Invited talk at “Differential display and related techniques for gene discovery” meeting, Cold Spring Harbor Laboratory, New York, USA, 1996.
74.Tsai, C.-J., G.K. Podila and V.L. Chiang. (1996). 3' cycle-labeled oligonucleotides with predictable length for primer extension and transgenic analysis. Nucleic Acids Research 24:5060-5062.
75.Ge, L. and V.L. Chiang. (1996). A full-length cDNA encoding trans-cinnamate 4-hydroxylase from developing xylem of Populus tremuloidesPlant Gene Register 96-075.
76.Zhang, X.-H. and V.L. Chiang. (1996). Single-stranded DNA ligation by T4 RNA ligase for PCR cloning of 5'-non-coding fragments and coding sequence of a specific gene. Nucleic Acids Research24:990-991.
77.(Book Chapter) Tsai, C.-J., Yu, J., Ge, L., Hu, W.-J., Kawaoka, A., Kumar, S., Podila, G.K., and Chiang, V.L. (1995). Transgenic aspen harboring antisense OMT gene and characterization of regulatory sequences of aspen OMT gene. In: Srebotnik, E., and Messner, K., eds., Biotechnology in the Pulp and Paper Industry: Recent Advances in Applied and Fundamental Research. Facultas‑Universitatsverlay, Vienna, Austria. pp. 301‑309.
78.(Book Chapter) Kawaoka, A. and V.L. Chiang. (1995). The molecular cloning and expression of syringyl and guaiacyl-specific hydroxycinnamate:CoA ligase from quaking aspen (Populus tremuloides). Proceedings of the Sixth International Conference on Biotechnology in the Pulp and Paper Industry. Vienna, Austria. June, 1995.
79.Bugos, R.C., V.L. Chiang, E.R. Campbell, G.K. Podila and W.H. Campbell. (1995). RNA isolation from plant tissues recalcitrant to extraction in guanidine. BioTechniques 19(5):734-737.
80.Tsai, C.-J., G.K. Podila and V.L. Chiang. (1995). Nucleotide sequence of a Populus tremuloides gene encoding bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase. Plant Physiology 107:1459.
81.Tsai, C.-J., G.K. Podila and V.L. Chiang. (1994). Agrobacterium-mediated transformation of quaking aspen (Populus tremuloides) and regeneration of transgenic plants. Plant Cell Reports 14:94-97.
82.Dwivedi, U.N., W.H. Campbell, J. Yu, R.S.S. Datla, R.C. Bugos, V.L. Chiang and G.K. Podila. (1994). Modification of lignin biosynthesis in transgenic Nicotiana through expression of an antisense O-methyltransferase gene from Populus. Plant Molecular Biology 26:61-71.
83.Popko, J.L., P. Murthy and V.L. Chiang. (1993). Differences in activity and substrate specificityof 4-hydroxycinnamate: CoA ligase and cinnamoyl oxidoreductase in xylem tissue of normal and compression wood of loblolly pine. Plant Physiology 102(1):53.
84.(Book Chapter) Funaoka, M., I Abe and V.L. Chiang. (1992). Nucleus exchange reaction. In: Methods in Lignin Chemistry. C.W. Dence and S.Y. Lin, Editors. Springer-Verlag, New York. Page 6.6.
85.Bugos, R.C., V.L. Chiang and W.H. Campbell. (1992). Characterization of bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase from aspen. Phytochemistry 31(5):1495-1498.
86.Bugos, R.C., V.L. Chiang and W.H. Campbell. (1991). cDNA cloning, sequence analysis and seasonal expression of lignin bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase of aspen. Plant Molecular Biology 17:1203-1215.
87.Lee, D.Y. and V.L. Chiang. (1991). Synthesis of 4-(3-hydroxyl-1-propenyl)-2-methoxyphenol-Bovine serum albumin conjugate. A novel hapten-protein conjugate for the preparation of anti-4-(3-hydroxyl-1-propenyl)-2-methoxyphenol antibodies. Tetrahedron Letters 32(39):5255-5258.
88.Chiang, V.L., J. Yu and R.C. Eckert. (1990). Isothermal kinetics of Kraft delignification. Journal of Wood Chemistry 10(3):293-310.
89.Chiang, V.L. and M. Funaoka. (1990). The response of guaiacyl-syringyl and guaiacyl lignins to Kraft delignification. Holzforschung 44(4):309-313.
90.Bugos, R.C., V.L. Chiang and W.H. Campbell. (1990). Cloning and expression of lignin bispecific O-methyltransferase. Plant Physiology 93:15.
91.Chiang, V.L. and M. Funaoka. (1990). The dissolution and condensation reactions of guaiacyl and syringyl units in residual lignin during Kraft delignification of sweetgum. Holzforschung 44(2):147-156.
92.(Book Chapter) Chiang, V.L. and M. Funaoka. (1989). Comparison of lignin fragmentation and condensation reactions in Kraft delignification of Douglas fir and sweetgum. In: Wood Processing and Utilization. J.F. Kennedy, G.O. Phillips and P.A. Williams, Editors. Ellis Horwood Limited. West Sussex, England. 43-48.
93.(Book Chapter) Chiang, V.L., J. Yu, R.C. Eckert and M. Rea. (1989). Isothermal reaction kinetics of Kraft delignification. In: Wood Processing and Utilization. J.F. Kennedy, G.O. Phillips and P.A. Williams, Editors. Ellis Horwood Limited. West Sussex, England. 105-110.
94.Chiang, V.L., D.D. Stokke and M. Funaoka. (1989). Lignin fragmentation and condensation reactions in middle lamella and secondary wall regions during Kraft pulping of Douglas fir. Journal of Wood Chemistry and Technology 9(1):61-83.
95.Chiang, V.L. and M. Funaoka. (1988). The formation and quantity of diphenylmethane-type structures in residual lignin during Kraft delignification of Douglas fir. Holzforschung 42(6):369-375.
96.Chiang, V.L., R.J. Puumala and H. Takeuchi. (1988). Comparison of softwood and hardwood Kraft pulping. TAPPI 71(9):173-176.
97.Chiang, V.L., G.D. Mroz, S.M. Shaler and D.D. Reed. (1987). Pulp production of a 16-year-old European larch stand. TAPPI 71:179-180.
98.Chiang, V.L., H.J. Cho, R.J. Puumala, R.E. Eckert and W.S. Fuller. (1987). The chemical consumption in Kraft pulping of Douglas fir, western hemlock and red alder. TAPPI 70(2):101-106.
99.Chiang, V.L., P. Cho and K.V. Sarkanen. (1986). Stabilization of western hemlock hemicelluloses with ammonium hydrosulfide for Kraft pulping. Paperi ja Puu 2:11.
100.Chiang, V.L. and K.V. Sarkanen. (1985). End-wise degradation of hydrocellulose in mildly alkaline solutions and its retardation by ammonia. Journal of Wood Chemistry and Technology 5(2):203-216.
101.Strand, S., J. Dykes and V.L. Chiang. (1984). Aerobic microbial degradation of glucoisosaccharinic acid. Applied Environmental Microbiology 47:268-271.
102.Chiang, V.L. and Kyosti V. Sarkanen. (1984). Kinetic study of end-group stabilization in hydrocellulose by hydrogen sulfide. J. of Wood Chemistry and Technology 4(1):1-18.
 
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