林盈仲副教授

 

中文名字:林盈仲
电话:919-389-6864
E-mailylin14@ncsu.edu
 
教育状况:
2010.072014.05     北卡罗来纳州立大学 (North Carolina State university),林木生物技术专业
研究方向:木质素生物合成讯息传导调控
2006.092008.09     台湾大学,理学硕士,植物科学专业
研究方向:淀粉生物合成调控
2002.092006.09     台湾清华大学,理学学士,生物学专业
 
学术研究及工作概况:
2010.07至今,美国北卡罗来纳州立大学自然资源学院林木生物技术研究中心,
导师姜立泉(Vincent L. Chiang) 教授。
主要研究方向:
1.首次发现调控木材合成主要转录因子的可变简切(Alternative splicing)以及此可变简切的功能与调控方式;
2.首次建立杨树木质部原生质体(xylem protoplast)分离与转化系统,并利用此技术解析木材形成过程中讯息传导途径,且建立整体调控网络;
3.首次利用杨树木质部原生质体进行染色质免疫共沉淀技术 (chromatin immunoprecipitation, ChIP)进行表观遗传解析;
4.以杨树为模式植物进行遗传转化,研究不同基因之间的体内调节机制,获取可应用化之遗传表型;
5.透过木材合成主要转录因子之亚细胞定位(subcellular localization)研究其功能及二聚体形成模式(Dimerization)
6.首次建立鞍树木质部原生质体(xylem protoplast)分离系统,进而研究鞍树抗寒抗冻机理;
7.首次建立杨树雷射演微切割技术(Laser-Capture Microdissection),并利用此技术剖析木质部不同细胞型态之基因表达
 
2013.12至今,东北林业大学,林木遗传育种国家重点实验室,兼职副教授
 
2006.092008.09,台湾大学植物科学所。
主要研究方向:
1.研究淀粉分支酶在淀粉生物合成中之功能以及调控机制;
2.利用扫描式(SEM)与穿透式(TEM)电子显微镜研究淀粉颗粒物理结构;
3.利用高效液相层析仪(HPLC)研究淀粉颗粒之化学结构;
4.建立微量淀粉定量法。
 
科研论文:
1.Chen, H.-C.*, Song, J.*, Wang, P.J.*, Lin, Y.-C., Ducoste, J., Shuford, M.C., Liu, J., Li Q., Shi, R., Nepomuceno, A., Isil, F., Muddiman, C.D., Williams, C., Sederoff, R.R., and Chiang L.C. (2014). Systems Biology of Lignin Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric acid: CoA Ligase (4CL) Protein Complex Formation, Regulation and Numerical Modeling. Plant Cell. (In press)
2.Lin, Y.C.*,Li, W.*, Li, Q.Z., Chen, H., Sederoff, R.R., and Chiang, V.L. (2014). A simple high throughput xylem protoplast system for studying wood formation. Nat. Protocols (Invited Submission, * Co-first author).
3.Li, W.*, Lin, Y.C.*, Li, Q.Z., Chen, H., Sederoff, R.R., and Chiang, V.L. (2014). A robust anti-transcription factor (TF) antibody-based chromatin immunoprecipitation (ChIP) protocol for studying TF direct targets in woody plants. Nat. Protocols(Invited Submission, * Co-first author).
4.Lin, Y.C.*, Li, W.*, Sun, Y.H., Kumari, S., Wei, H.R., Li, Q.Z., Tunlaya-Anukit, S., Sederoff, R.R., and Chiang, V.L. (2013). SND1 transcription factor-directed quantitative functional hierarchical genetic regulatory network in wood formation in Populus trichocarpa. Plant Cell DOI: 10.1105/tpc.113.117697.(* Co-first author).
5.Li Q.*, Lin Y.C.*, Sun Y. H., Song J., Chen H., Zhang X. H., Sederoff R. R., and Chiang V. L. (2012). Splice variant of the SND1 transcription factor is a dominant negative of SND1 members and their regulation in Populus trichocarpa. PNAS.109, 14699-14704. (* Co-first author).
6.Wang J.P., Shuford C.M., Li Q., Song J., Lin Y.C., Sun Y.H., Chen H.C., Williams C.M., Muddiman D.C., Sederoff R.R., Chiang V.L. (2012). Functional redundancy of the two 5-hydroxylases in monolignol biosynthesis of Populus trichocarpa: LC-MS/MS based protein quantification and metabolic flux analysis. Planta. 236(3):795-808.
 
学术会议:
1.Lin, Y.C., Li, W., Sun, Y.H., Wei, H.R., Li, Q.Z., Sederoff R. and Chiang, V.L. Populus trichocarpa differentiating xylem protoplasts as a model system for studying transcriptional regulatory hierarchy in wood formation. The 2013 ASPB meeting, Providence, Rhode Island, USA, July, 20, 2013.
2.Li Q., Lin Y.C., Li, W., Sun Y. H., Song J., Chen H., Zhang X. H., Sederoff R. R., and Chiang V. L. Splice variant of the SND1 transcription factor is a dominant negative of SND1 members and their regulation in Populus trichocarpa. The 2013 DOE meeting, Washington DC, USA, February, 2013.
3.Chen H.C., Li Q., Shuford M. C., Liu J., Lin Y.C., Muddiman D., Sederoff R., and Chiang V.L. Cytochrome P450 monooxynenases form proteins complexes to regulation key hydroxylation steps in monolignol biosynthesis. Plant & Animal Genome XX Conference, San Diego, CA, January 2012)