李成浩

李成浩博士,教授,博士研究生导师

E-mailchli0@163.com

  话:0451-82190607-11 

  真:0451-82190607-11 


研究方向:林木基因工程育种和林木细胞工程

研究领域

林木基因工程育种

采用分子生物学技术,克隆控制杨树和落叶松生长发育及抗逆等性状的调控基因和功能基因,通过转基因等方法验证其功能,揭示基因的作用机理,为通过生物技术定向改造林木的性状提供技术支持和理论依据。

林木细胞工程

利用体细胞胚发生途径建立落叶松和红皮云杉等针叶树种的无性繁殖体系,开展体细胞胚的悬浮培养和生物反应器培养苗木工厂化生产体系建立研究


学习工作经历

教育背景:

1993年于延边农学院获学士学位;
2001
年于韩国江原大学资源植物学科,获硕士学位;
2004
年于韩国江原大学农学学科,获博士学位。

     

工作经历:

2004—2009年,东北林业大学林学院,副教授;

2009  今, 东北林业大学林学院,教授。


承担科研项目

1)主持国家自然科学基金面上项目 “刺五加体细胞胚在生物反应器中的生长动力学及抗氧化特性”;时间:20072009;项目编号:30671701

2)主持国家高新技术研究发展计划(863计划)项目子课题“落叶松抗逆基因工程研究”;时间:20132017;项目编号:2013AA102704-02

3)主持国家自然科学基金面上项目 “毛果杨HD-Zip转录因子调控不定根发生和低氮胁迫抗性的分子机理研究”;时间:20172010;项目编号:31670668

 

  利:

1)李成浩,王伟达,张含国,刘桂丰,杨静莉(2011)一种长白落叶松离体培养不定芽诱导植株再生的方法。中国发明专利号:ZL200810063950.3

2)李成浩,杨静莉,刘立琨,李俊秀,周晨光,陈爱华(2011)一种西兰花体细胞胚诱导植株再生的方法。中国发明专利号:ZL200910305848.4

3)李成浩,刘宝光,张含国,刘桂丰(2010)一种红皮云杉体细胞胚诱导植株再生的方法。中国发明专利号:ZL200810064037.5

4)李成浩,杨传平,刘桂丰,牛遇达,赵波,韩君(2008)不依赖植物激素的刺五加体细胞胚的诱导和植株再生方法。中国发明专利号:ZL20061009783

5)李成浩,杨传平,刘桂丰,牛遇达,赵波,韩君(2008)北五味子体细胞胚发生和植株再生方法。中国发明专利号:ZL200610009782.0

6)李成浩,杨静莉,许雪梅 2013)一种农杆菌介导的培育转基因旱柳植株的方法。中国发明专利号:ZL201310021280.X

 

获奖情况:

1)张含国,李成浩,康迎昆,袁桂华,韩家永。“杂种落叶松优良家系选育与扩繁技术”,黑龙江省科技进步二等奖,2013

 

发表论文:

1Zhou Chenguang, Chenghao Li* (2016) A R2R3-MYB transcription factor BpMYB106 of birch (Betula platyphylla) confers increased photosynthesis and growth rate through up-regulating photosynthetic gene expression. Front Plant Sci 7:315 (SCI, *通讯作者)

2Zhanchao Wang, Quangang Liu, Hanzeng Wang, Haizhen Zhang, Xuemei Xu, Chenghao Li*, Chuanping Yang* (2016) Comprehensive analysis of trihelix genes and their expression under biotic and abiotic stresses in Populus trichocarpa. Sci Rep 6: 36274 (SCI, *通讯作者)

3Li Dandan, Xu Xuemei, Hu Xiaoqing, Liu Quangang, Wang Zhanchao, Zhang Haizhen, Wang Han, Wei Ming, Wang Hanzeng, Liu Haimei, Chenghao Li* (2015) Genome-wide identification and expression profiling of the copper transporter gene family in Populus trichocarpa. Front Plant Sci 6:1149 (SCI, *通讯作者)

4Hu Xiaoqing, Yang Jingli, Chenghao Li* (2015) Transcriptomic response to nitric oxide treatment in Larix olgensis Henry. Int J Mol Sci 16:1–16 (SCI, *通讯作者)

5Zhang Haizhen, Yang Jingli, Wang Weida, Li Dandan, Hu Xiaoqing, Wang Han, Wei Ming, Liu Quangang, Wang Zhanchao, Chenghao Li* (2015) Genome-wide identification and expression profiling of the copper transporter gene family in Populus trichocarpa. Plant Physiol Biochem 97:451–460 (SCI, *通讯作者)

6Yang Jingli, Li Kun, Zheng Wei, Zhang Haizhen, Cao Xudong, Lan Yunxiang, Yang Chuangping*, Chenghao Li* (2015) Characterization of early transcriptional responses to cadmium in the root and leaf of Cd-resistant Salix matsudana Koidz. BMC Genomics 16:705 (SCI, *通讯作者)

7Liu Quangang, Wang Zhanchao, Xu Xuemei, Zhang Haizhen, Chenghao Li* (2015) Genome-wide analysis of C2H2 zinc-finger family transcription factors and their responses to abiotic stresses in poplar. PLoS ONE 10(8):1–25 (SCI, *通讯作者)

8Wang Zhanchao, Liu Quangang, Xu Xuemei, Yang Jingli, Zhang Teng, Chenghao Li* (2015) Identification of microRNAs associated with male flower bud development of Populus simonii × Populus nigra. Trees-Structure and Function 29:1329–1339 (SCI, *通讯作者)

9Liu Quangang, Yang Jingli, Wang Zhanchao, Xu Xuemei, Mao Xuliang, Li Dandan, Hu Xiaoqing, Jin Dongchun, Chenghao Li* (2015) Genome-wide classification, identification and expression profile of the C3HC4-type RING finger gene family in Poplar (Populus trichocarpa). Plant Mol Biol Rep 33:1740–1754 (SCI, *通讯作者)

10Yang Jingli, Chen Zhuo, Wu Songquan, Cui Yi, Zhang Lu, Dong Hao, Yang Chuanping*, Li Chenghao Li* (2015) Overexpression of the Tamarix hispida ThMT3 gene increases copper tolerance and adventitious root induction in Salix matsudana Koidz. Plant Cell Tiss Org Cult 121:469–479 (SCI, *通讯作者)

11Chenguang Zhou, Likun Liu, Chenghao Li* (2014) Microarray analysis of siberian ginseng cyclic somatic embryogenesis culture systems provide insight into molecular mechanisms of embryogenic cell cluster generation. PLoS ONE 9(4): e94959 (SCI, *通讯作者)

12Shicheng Zhao, Pham Anh Tuan, Jae Kwang Kim, Woo Tae Park, Yeon Bok Kim, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Jingli Yang, Chenghao Li*, Sang Un Park* (2014) Molecular characterization of carotenoid biosynthetic genes and carotenoid accumulation in Lycium chinense. Molecules, 19: 1125011262 (SCI, *通讯作者)

13Xuliang Mao, Xuemei Xu, Haizhen Zhang, Jingli Yang, Zhanchao Wang, Chenghao Li* (2014) Comparison and analysis of transcriptome profiles of haploid and diploid Populus by digital gene expression. Plant Omics 7(4):244252 (SCI, *通讯作者)

14Yang JL, JS Yi, Chuanping Yang*, Chenghao Li* (2013) Agrobacterium tumefaciens–mediated genetic transformation of Salix matsudana Koidz. using mature seeds. Tree Physiol 33: 628–639  (SCI, *通讯作者)

 15Jingli Yang, Bo Zhao, Yeonbok Kim, Chenguang Zhou, Chunyan Li, Yunlin Chen, Haizhen Zhang, Chenghao Li* (2013) Agrobacterium tumefaciens- mediated transformation of Phellodendron amurense Rupr. using mature-seed explants. Mol Biol Rep 40:281–288 (SCI, *通讯作者)

16Zhanchao Wang, Haizhen Zhang, Jingli Yang, Yunlin Chen, Xuemei Xu, Xuliang Mao, Chenghao Li* (2013) Phylogenetic, expression, and bioinformatic analysis of the ABC1 gene family in Populus trichocarpa. Scientific World J Article ID 785070, 1–11 (SCI, *通讯作者)

17Yang JL, Wu SQ, Chenghao Li* (2013) High efficiency secondary somatic embryogenesis in Hovenia dulcis Thunb. through solid and liquid cultures. Scientific World J Article ID 718754, 1–6 (SCI, *通讯作者)

18Jingli Yang, Shicheng Zhao, Changyeon Yu, Chenghao Li* (2013) Large-scale plantlet conversion and ex vitro transplantation efficiency of Siberian ginseng by bioreactor culture. Scientific World J Article ID 829067 (SCI, *通讯作者)

19Yunlin Chen, Jingli Yang, Zhanchao Wang, Haizhen Zhang, Xuliang Mao, Chenghao Li* (2013) Gene structures, classification and expression models of the DREB transcription factor subfamily in Populus trichocarpa. Scientific World J Article ID 954640 (SCI, *通讯作者)

20Haizhen Zhang, Jingli Yang, Yunlin Chen, Xuliang Mao, Zhanchao Wang, Li Chenghao* (2013) Identification and expression analysis of the heat shock transcription factor family in Populus trichocarpa. Plant Omics 6:415-424 (SCI, *通讯作者)

21SC Zhao, PA Tuan, XH Li, YB Kim, JL Yang, Chenghao Li*, Sangun Park (2013) Identification of phenylpropanoid biosynthetic genes and phenylpropanoid accumulation by transcriptome analysis of Lycium chinense. BMC Genomics 14:802 (SCI, *通讯作者)

22Jingli Yang, Chenguang Zhou, Lihui Liu, Du Jia, Zhiyang Yin, Myongjo Kim, Changyeon Yu, Li ChengHao* (2012) High conversion frequency of germinated somatic embryos of Siberian ginseng (Eleutherococcus senticosus Maxim) using a bubble column bioreactor. Plant Cell Tiss and Org Cult 110:289–298 (SCI, *通讯作者)

23Du Jia, Yang Jingli, Li Chenghao* (2012) Advances in metallotionein studies in forest trees. Plant Omics 5:46–51 (SCI, *通讯作者)

24Yang JL, Wang YC, Liu GF, Yang CP, Li Chenghao* (2011) Tamarix hispida metallothionein-like ThMT3, a reactive oxygen species scavenger, increases tolerance against Cd2+, Zn2+, Cu2+, and NaCl in transgenic yeast. Mol Biol Rep 38:1567–1574 (SCI, *通讯作者)

25Yang Jingli, Niu Yuda, Liu Guifeng, Yang Chuanping, Li Chenghao* (2011) Induction of somatic embryogenesis from female flower buds of elite Schisandra chinensis. Plant Cell Tiss Org Cult 106:391–399 (SCI, *通讯作者)

26Zhou CG, Yang JL, Liu LK, Liang CN, Xia DA, Li Chenghao*(2011) Research progress in somatic embryogenesis of Siberian ginseng (Eleutherococcus senticosus Maxim.) J Med Plants Res 5: 7140–7145 (SCI, *通讯作者)

27Yang JL, Zhou CG, Zhao B, Jin CL, Zhang T, Li Chenghao* (2011) Rapid direct adventitious shoot organogenesis and plant regeneration from mature seed explants of Phellodendron amurense Rupr. J Med Plants Res 5:4560–4565 (SCI, *通讯作者)

28Yang JingLi, Seong EunSoo, Kim MyongJo, Ghimire BK, Kang WonHee, Yu ChangYeon, Chenghao Li* (2010) Direct somatic embryogenesis from pericycle cells of broccoli (Brassica oleracea L. var. italica) root explants. Plant Cell Tiss Org Cult 100:49–58 (SCI, *通讯作者)

29Chen AiHua, Yang JingLi, Niu YuDa, Yang ChuanPing, Liu GuiFeng, Yu ChangYeon, Li Chenghao* (2010) High-frequency somatic embryogenesis from germinated zygotic embryos of Schisandra chinensis and evaluation of the effects of medium strength, sucrose, GA3, and BA on somatic embryo development. Plant Cell Tiss and Org Cult 102:357–364 (SCI, *通讯作者)

30Yang JL, Zhao B, Seong ES, Kim MJ, Kang WH, Kim NY, Yu CY, Li Chenghao* (2010) Callus induction and high-efficiency plant regeneration via somatic embryogenesis in Papaver nudicaule L., an ornamental medicinal plant. Plant Biotechnol Rep 4:261–267 (SCI, *通讯作者)

31Li ChengHao, Liu BaoGuang, Kim TaeDong, Moon HK, Choi YongEui (2008) Somatic embryogenesis and plant regeneration in elite genotypes of Picea koraiensis. Plant Biotechnol Rep 4:259–265 (SCI)

 

专著

李成浩. 红皮云杉和杂种落叶松离体培养再生体系的建立研究. 东北林业大学出版社. 2009